Characteristics of Potential Protein Biomarkers Extracted with 10% TCA from Blood Serum of Non-Hodgkin’s Lymphoma and Multiple Myeloma Patients

Blood serum has been extensively explored as a source of the bio-markers [1, 2].  For concentration of minor protein(s) and depletion of abundant blood serum proteins a 2,2,2-trichloroacetic acid (TCA) precipitation procedure is frequently applied [3]. However, a significant amount of proteins may be present in the TCA extracts, and these proteins are often not studied.Recently, we have shown that a TCA-extracted fraction obtained from blood serum of the multiple sclerosis (MS) patients contains two proteins that were identified by the MALDI TOF/TOF as blood serum albumin (BSA) and a short form of the unconventional myosin lc (sMyo1C) [4]. We also demonstrated that the TCA-extracted fractions isolated from blood serum of the MS patients contain IgGs and/or their heavy chains [5]. These proteins have not been detected in the TCA-extracted fractions isolated from blood serum of healthy human donors and patients with the systemic lupus erythematosus or the rheumatoid arthritis.Here we report that the TCA-soluble fraction isolated from blood serum of the non-Hodgkin’s lymphoma contains sMyo1C that have been earlier detected in blood serum of the MS patients, while the blood serum of the multiple myeloma patients, in addition to that protein and albumin, also contains the IgG polypeptides.